Bench-to-bedside review: Diaphragm muscle function in disuse and acute high-dose corticosteroid treatment
© BioMed Central Ltd 2009
Published: 8 September 2009
Critically ill patients may require mechanical ventilatory support and short-term high-dose corticosteroid to treat some specific underlying disease processes. Diaphragm muscle inactivity induced by controlled mechanical ventilation produces dramatic alterations in diaphragm muscle structure and significant losses in function. Although the exact mechanisms responsible for losses in diaphragm muscle function are still unknown, recent studies have highlighted the importance of proteolysis and oxidative stress. In experimental animals, short-term strategies that maintain partial diaphragm muscle neuromechanical activation mitigate diaphragmatic force loss. In animal models, studies on the influence of combined controlled mechanical ventilation and short-term high-dose methylprednisolone have given inconsistent results in regard to the effects on diaphragm muscle function. In the critically ill patient, further research is needed to establish the prevalence and mechanisms of ventilator-induced diaphragm muscle dysfunction, and the possible interaction between mechanical ventilation and the administration of high-dose corticosteroid. Until then, in caring for these patients, it is imperative to allow partial activation of the diaphragm, and to administer the lowest dose of corticosteroid for the shortest duration possible.
Through a complex integration of feedback signals, the respiratory center generates signal output to the diaphragm muscle leading to its rhythmic contractions. Some critically ill patients, including those with acute insults to the respiratory center, upper spinal cord, bilateral phrenic nerves or neuromuscular junction or those receiving neuromuscular paralysis - for instance, patients with acute respiratory distress syndrome  - must be supported with the application of controlled mechanical ventilation (CMV), where the ventilator takes full control of the act of breathing and the respiratory muscles do not contract. In addition to mechanical ventilation, some critically ill patients - such as victims of acute spinal cord injury , of lung transplant rejection , of hematologic malignancy  and of status asthmaticus  - may require administration of high doses of corticosteroids.
The extent to which CMV  or (short-term) high-dose corticosteroid administration  negatively impacts diaphragm muscle function has been demonstrated in experimental animals. In critically ill patients, however, the presence of confounding factors (for example, sepsis, malnutrition, hyperglycemia) makes it difficult to determine the extent to which diaphragm muscle dysfunction is attributable to disuse or high-dose corticosteroid alone, or in combination. The reported studies suggest that deleterious effects in the diaphragm occurred with both diaphragm muscle disuse  and possibly with the administration of high-dose corticosteroid , leading to difficulty weaning from mechanical ventilation.
The goal of the present article is to address two key issues: to identify the underlying mechanisms responsible for the loss of diaphragmatic function that occur as a result of CMV and acute high-doses of corticosteroids; and to determine the evidence of diaphragm muscle impairment in humans, and the potential approaches for protecting the diaphragm muscle.
Mechanisms of diaphragm muscle dysfunction with disuse
Several animal studies have demonstrated that CMV reduces the contractile function of previously healthy diaphragm muscle with intact neural outflow tract and neurotrophic influences, a condition referred to as ventilator-induced diaphragmatic dysfunction (VIDD) [6, 10, 11]. The impairment occurs fairly rapidly and is progressive. In rabbits, compared with a control, the diaphragmatic force-generating capacity declined by 25% after 24 hours of CMV, and by 44% after 72 hours of CMV . In rats, the rate of diaphragmatic force loss was more profound than that in rabbits (46% after only 24 hours of CMV) . The deleterious effects of CMV-induced diaphragmatic dysfunction are not exclusive to rodents [6, 12, 13]. It is plausible that the diaphragm's lack of constant rhythmic contractions makes it susceptible to functional derangement with inactivity, even when the inactivity is of short duration.
CMV induces diaphragm muscle inactivity via phrenic inhibition. Superimposed to the already inactive diaphragm from CMV application, the administration of cisatracurium - a benzylisoquinolinium nondepolarizing paralytic - does not exacerbate the force loss . In contrast, rocuronium - an aminosteroid nondepolarizing paralytic - worsens diaphragmatic force loss . Testelmans and colleagues postulated that this difference is related to rocuronium's corticosteroid molecular structure .
Studies assessing the mechanisms of CMV-induced diaphragm muscle dysfunction have attributed the dysfunction predominantly to increased proteolysis [16–18] with and without the requirement of oxidative stress [19, 20]. Proteolysis is conducive to myofibrilar disruption and/or atrophy (reduced cross-sectional area) . It should be noted that impairment in excitation-contraction coupling has not been investigated systematically. Impaired excitation-contraction coupling (that is, a decrease in sarcolemma resting membrane action potential and/or sarcoplasmic reticulum Ca2+ release capacity) leads to reduced force development .
Excessive oxidative stress results from a decrease in anti-oxidant buffering capacity and/or the overproduction of reactive oxygen species (ROS) . CMV compromises anti-oxidant defenses [24, 25]. CMV decreases the total anti-oxidant capacity and glutathione (a nonenzymatic antioxidant) concentrations [24, 25]. The effects of CMV on enzymatic antioxidant (for example, glutathione peroxidase) are variable. For instance, in rats the glutathione-peroxidase activity decreases after 12 hours of CMV , while in piglets the activity remains unchanged after 3 days of CMV .
Overproduction of ROS can occur even following short periods of CMV. For instance, Zergeroglu and colleagues observed significant elevations in ROS levels after only 6 hours of CMV . Importantly, the elevated ROS levels were associated with atrophy of all fiber types and diaphragmatic force loss after 12 to 18 hours of CMV [16, 27]. The trigger for the increased oxidative stress remains unknown.
The nitric oxide synthase pathway does not seem to be involved in VIDD . Conversely, Whidden and coworkers recently reported that the xanthine oxidase pathway contributes to the oxidative damage of diaphragm muscle . This hypothesis was supported by the observation that administration of oxypurinol, a xanthine-oxidase inhibitor, partially attenuates diaphragmatic dysfunction after 12 hours and 18 hours of CMV . Markers of protein and lipid peroxidation, protein carbonyls and 4-hydroxynoneal, respectively, are also suppressed with the administration of oxypurinol. While xanthine oxidase contributes to diaphragm muscle force loss, xanthine-oxidase inhibition does not attenuate CMV-induced diaphragm muscle atrophy , suggesting that other oxidative stress pathways may be involved in the atrophic process.
In addition to xanthine oxidase, McClung and colleagues demonstrated the role of the NADPH oxidase pathway in producing oxidative damage in the diaphragm . In rats receiving 18 hours of CMV, apocynin (an inhibitor of NADPH oxidase) attenuated diaphragm muscle dysfunction, prevented atrophy of all myofiber types, and prevented CMV-induced reduction in glutathione. Furthermore, apocynin not only suppressed calpain-1 and caspase-3 activation, but in fact increased calpastatin, an endogenous calpain inhibitor. Among the oxidative stress pathways, however, the mitochondrial oxidant-generating pathway is key in the development of oxidative stress damage of the diaphragm with CMV . Recently, Kavazis and colleagues demonstrated that mitochondriae are a major source of ROS production associated with mitochondrial oxidative damage and with mitochondrial respiratory dysfunction .
Consistent with the mitochondrial oxidant-generating pathway, an earlier study from the same laboratory demonstrated elevated intracellular oxidant production with CMV . The latter was estimated from the intracellular increased emission of dichlorodihydrofluorescein dye, a chemical that fluoresces upon reaction with oxidative species . The enhanced production of lipid and protein oxidation markers underscores the elevated oxidative stress . Lipid oxidation may result in cellular membrane dysfunction (that is, decreased Ca2+ ATPase activity) and may delay Ca2+ removal from the cytosol, causing its accumulation within the cytosol itself . The elevated Ca2+ concentration in the cytosol can activate calpain, the Ca2+-dependent proteases . In fact, calpain-1 is an absolute requirement for oxidative stress-induced myofiber atrophy . This contention was supported in experiments with hydrogen-peroxide-incubated myotube cell cultures. Hydrogen peroxide induced myotube atrophy. In contrast, calpain-1 RNA interference (gene knocked out) completely prevents the atrophy .
All of the major proteolytic systems are responsible for CMV-induced proteolysis; these include lysosomal proteases , calcium-dependent proteolysis or calpains , caspase-3 , and the ATP-dependent ubiquitin proteasome . Lysosomal proteases are primarily responsible for proteolysis of extracellular proteins and cell surface receptors . Calpain proteases are involved in the cleavage of cytoskeletal proteins (for example, titin, nebulin, desmin) that anchor contractile elements of myosin to actin . Caspases (endoproteases responsible for the final execution of cell death) including caspase-3 proteases induce DNA fragmentation, induce myonuclear apoptosis, and cleave actomyosin complexes [34, 37], whereas the ubiquitin-proteasome pathway degrades the myofilament actin and myosin .
Lysosomal proteases and calpains play a significant role in diaphragmatic dysfunction with inactivity . Pretreatment with leupeptin - an inhibitor of lysosomal thiol proteases and calcium-activated proteases - completely prevents the CMV-induced reduction in diaphragmatic force and atrophy . Likewise, caspase-3 vitally contributes in the detrimental effects of CMV on the diaphragm . Treatment of animals with caspase inhibitor prevents myonuclei loss, DNA fragmentation, and myofiber atrophy .
The ubiquitin-proteasome pathway is responsible for most muscle protein degradation . The ubiquitin-proteasome system, however, does not break down complexes of proteins contained in myofibrils. One or more other proteases are required in the initial process to release myofilament contractile proteins (that is, actin and myosin) for the ubiquitin-proteasome system to degrade those proteins . The binding of ubiquitin to protein substrates requires ubiquitin-activating enzyme (E1), ubiquitin-carrier enzyme (E2), and ubiquitin ligases (E3)  (Figure 2). Two of the E3 ligases - the muscle atrophy F-box (MAFbox; atrogin-1, atrogenes) and muscle ring finger-1 (MuRF1) genes - are overexpressed in various models of skeletal muscle atrophy . Similarly, MAFbox and MuRF1 are upregulated during CMV-induced diaphragm muscle inactivity [11, 19].
Influence of neuromechanical activation on diaphragmatic function
In another study, when spontaneous breathing for 5 minutes or 60 minutes was interposed during 24 hours of CMV four times a day, the diaphragmatic force-generating capacity decreased by an average of 19% and decreased by 28% with continuous CMV, respectively . Although the protective effects of a brief duration of diaphragm muscle activation on functional loss were modest (~9%), the activation prevents diaphragm muscle atrophy. Futier and colleagues recently demonstrated that maintaining diaphragmatic activation with pressure support ventilation for 18 hours did not augment proteolysis . Protein carbonyls (markers of oxidative stress), however, were elevated to the same extent as with CMV. Unfortunately, measures of diaphragmatic function were not performed, and whether pressure support ventilation preserves diaphragmatic force therefore remains unknown .
Evidence of CMV-induced diaphragmatic dysfunction in humans and a potential approach to prevention
In humans, the degree of diaphragm muscle activation that will preserve force remains unknown. In a prospective trial, critically ill patients who were predicted to receive mechanical ventilation for longer than 72 hours were randomized into controls (n = 13) and those receiving inspiratory muscle training (IMT) (n = 12) from the onset of mechanical ventilation . A threshold load was used for the IMT by setting the ventilator pressure-triggering sensitivity at 10% or 20% of the initial maximum inspiratory pressure (PImax), whichever was tolerated, and was applied twice daily for 5 minutes. When the patient tolerated the initial load, the next training duration was increased by 5 minutes up to a maximum of 30 minutes. Afterwards, the load was increased by 10% increments until 40% of the initial PImax value was attained. Sedation and analgesia with intravenous midazolam and fentanyl, respectively, were administered. The IMT session was aborted according to specified criteria. Weaning with decreasing pressure support was initiated once the patient met the weaning criteria. The initial and final PImax values in the training group were similar to those of the control group (initial, -51 cmH2O vs. -48 cmH2O; final, -56 cmH2O vs. -55 cmH2O, respectively). The duration of mechanical ventilation or of the weaning trial was similar for both groups, with a trend toward a shorter duration for the IMT group compared with the control group (mean duration of mechanical ventilation, 8.6 days vs. 9.8 days; mean duration of weaning trial, 23 hours vs. 31 hours, respectively).
The lack of IMT benefits may be due to the small sample size . It is also conceivable that the magnitude of the stimulus for IMT, as a percentage of the PImax, in the critically ill patients (that is, the threshold load applied, and/or session frequency and duration) was inadequate to elicit a physiological training effect. Measurements of PImax in the critically ill patients are challenging and highly dependent on patient volitional effort and the methods of measurement. Alternatively, in view of the complexity of the underlying mechanisms of CMV-induced diaphragmatic dysfunction, diaphragm muscle conditioning alone is inadequate, and pharmacological intervention may be required to mitigate diaphragm muscle weakness.
Mechanisms of the interactive effects of mechanical ventilation and short-term high-dose corticosteroid on diaphragm muscle dysfunction
Short-term high-dose corticosteroid has been administered for its anti-inflammatory and immunosuppressive effects in critically ill patients [9, 45]; the treatment might be responsible for the development of acquired paresis in the critically ill patient, referred to as critical-illness myopathy . Patients may receive both mechanical ventilation and short-term high-dose corticosteroid, yet the effects of acute high-dose corticosteroid alone or its interaction with mechanical ventilation is not well understood.
We recently studied the temporal relationship (1 to 3 days of 80 mg/kg/day intramuscularly) and dose-response effects (3 days of 80 mg/kg/day vs. 10 mg/kg/day intramuscularly) of methylprednisolone (MP) treatment in rabbits . MP induced a progressive decline in diaphragmatic force by 19%, 24%, and 34% after 1 day, 2 days, and 3 days, respectively. The decline in diaphragmatic force correlated with the degree of abnormal myofibril volume density. Low-dose MP (10 mg/kg/day, but a high dose by clinical standards) decreased diaphragmatic force modestly, by 12%. The suppression of IGF-1 and upregulation of MAFbox mRNA were independent of the MP dose . Both high-dose and low-dose MP decreased IGF-1 by 35%, and increased MAFbox mRNA by threefold . Clearly, short-term high doses of MP in spontaneously breathing animals produced detrimental effects on the diaphragm. The combination of both CMV and high-dose MP is therefore expected to aggravate the decline in diaphragmatic force compared with either CMV or MP alone.
Interestingly, Maes and colleagues demonstrated in rats that 24 hours of combined CMV and high-dose MP (80 mg/kg/day intramuscularly) preserved the diaphragmatic force compared with CMV alone . The mechanism by which MP prevented diaphragmatic force loss was via inhibition of calpain activity. Our preliminary data  in rabbits contrast with those of Maes and colleagues. After 2 days of combined MP (60 mg/kg/day intravenously) plus CMV, MP plus AMV, or MP plus continuous positive airway pressure, the diaphragmatic force decreased from that without MP by 10%, 16% and 18% from the average values of 16.1 Newton/cm2, 22.6 Newton/cm2, and 23.3 Newton/cm2 with CMV, AMV, and continuous positive airway pressure alone, respectively . The diaphragmatic force with the combined CMV and MP approach was not significantly different from that with CMV alone. This suggests that both CMV and MP share common mechanisms for the decrease in diaphragmatic force. It is unclear whether the discrepancy between our preliminary results  and those of Maes and colleagues  is related to species differences or to the duration of MP treatment.
Evidence of methylprednisolone-induced diaphragmatic dysfunction in humans and a potential approach to prevention
As with CMV, the extent to which acute, high-dose MP could contribute to diaphragm muscle weakness in critically ill patients is difficult to determine. This difficulty stems from the many confounding factors in these patients, and from the lack of data on functional or structural alterations in humans. Indirect data, however, suggest that such interaction may occur in critically ill patients .
First, in a prospective study of critically ill patients receiving mechanical ventilation for longer than 7 days, De Jonghe and colleagues reported a strong association between the occurrence of neuromyopathy and the administration of corticosteroids . Second, in patients with acute spinal cord injury, the administration of recommended high-dose MP for 48 hours resulted in paraspinal muscle necrosis and type II fiber atrophy in four out of five patients . Three of the patients remained ventilator dependent at discharge from the Spinal Cord Injury Center despite the relatively low level of injury . Finally, among 26 patients with chronic obstructive pulmonary disease who received mechanical ventilation and MP (240 mg/day), nine (35%) patients developed myopathy of the extremities - a condition associated with higher total doses of MP treatment (1,649 mg vs. 979 mg), with prolonged mechanical ventilation, and with prolonged hospital length of stay .
Whether a dose and duration of corticosteroids that confers beneficial anti-inflammatory effects and yet preserves diaphragm muscle integrity/function does exist remains unknown. More research is necessary to dissect the underlying mechanisms of the effects of corticosteroid on the diaphragm, particularly its interaction with mechanical ventilation. Because of the corticosteroid dose-response effects in both animal studies  and human studies , clinicians must carefully weigh the risks and benefits ratio, and must use the lowest corticosteroid dose for the shortest duration possible.
In laboratory animals the mechanisms responsible for VIDD have been the focus of intense investigation. Unfortunately, the triggering factor(s) for enhanced proteolysis in VIDD remain unknown. Similarly, the contribution of excitation-contraction coupling and the degree or duration of neuromechanical activation for preventing diaphragmatic force loss are unknown. Whether the benefits of AMV depend on the level of diaphragmatic activity or whether the benefits cease with time remains unclear. Diaphragm muscle conditioning using noninvasive phrenic nerve stimulation is a potential strategy for preventing VIDD that remains to be explored. In animal studies, treatment with specific inhibitors to the signaling cascade involved in proteolysis completely preserves diaphragm muscle function. Whether a similar strategy should be attempted in patients remains to be determined.
protein kinase-B serine/threonine kinase
assist-control mechanical ventilation
controlled mechanical ventilation
insulin-like growth factor-1
inspiratory muscle training
muscle atrophy F-box
muscle ring finger-1
nicotinamide adenine dinucleotide phosphate
maximal inspiratory pressure
reactive oxygen species
ventilator-induced diaphragmatic dysfunction.
The present work was supported by grants from the Department of Veterans Affairs Medical Research Service (to CSHS) and the National Institute of Arthritis and Musculoskeletal and Skin Diseases AR-46856 (to VJC). We thank Ercheng Zhu, Ph.D. for generating the data presented in Figure 4.
- Gainnier M, Roch A, Forel JM, Thirion X, Arnal JM, Donati S, Papazian L: Effect of neuromuscular blocking agents on gas exchange in patients presenting with acute respiratory distress syndrome. Crit Care Med 2004, 32: 113-119. 10.1097/01.CCM.0000104114.72614.BCView ArticlePubMed
- Bracken MB, Shepard MJ, Holford TR, Leo-Summers L, Aldrich EF, Fazl M, Fehlings M, Herr DL, Hitchon PW, Marshall LF, Nockels RP, Pascale V, Perot PL Jr, Piepmeier J, Sonntag VK, Wagner F, Wilberger JE, Winn HR, Young W: Administration of methylprednisolone for 24 or 48 hours or tirilazad mesylate for 48 hours in the treatment of acute spinal cord injury. Results of the Third National Acute Spinal Cord Injury Randomized Controlled Trial. National Acute Spinal Cord Injury Study. JAMA 1997, 277: 1597-1604. 10.1001/jama.277.20.1597View ArticlePubMed
- Nava S, Fracchia C, Callegari G, Ambrosino N, Barbarito N, Felicetti G: Weakness of respiratory and skeletal muscles after a short course of steroids in patients with acute lung rejection. Eur Respir J 2002, 20: 497-499. 10.1183/09031936.02.01732002View ArticlePubMed
- Jagannath S: Treatment of myeloma in patients not eligible for transplantation. Curr Treat Options Oncol 2005, 6: 241-253. 10.1007/s11864-005-0007-0View ArticlePubMed
- Kaplan PW, Rocha W, Sanders DB, D'Souza B, Spock A: Acute steroid-induced tetraplegia following status asthmaticus. Pediatrics 1986, 78: 121-123.PubMed
- Sassoon CSH, Caiozzo VJ, Manka A, Sieck GC: Altered diaphragm contractile properties with controlled mechanical ventilation. J Appl Physiol 2002, 92: 2585-2595.View ArticlePubMed
- Sassoon CS, Zhu E, Pham HT, Nelson RS, Fang L, Baker MJ, Caiozzo VJ: Acute effects of high-dose methylprednisolone on diaphragm muscle function. Muscle Nerve 2008, 38: 1161-1172. 10.1002/mus.21048View ArticlePubMed
- Levine S, Nguyen T, Taylor N, Friscia ME, Budak MT, Rothenberg P, Zhu J, Sachdeva R, Sonnad S, Kaiser LR, Rubinstein NA, Powers SK, Shrager JB: Rapid disuse atrophy of diaphragm fibers in mechanically ventilated humans. N Engl J Med 2008, 358: 1327-1335. 10.1056/NEJMoa070447View ArticlePubMed
- Qian T, Guo X, Levi AD, Vanni S, Shebert RT, Sipski ML: High-dose methylprednisolone may cause myopathy in acute spinal cord injury patients. Spinal Cord 2005, 43: 199-203. 10.1038/sj.sc.3101681View ArticlePubMed
- Powers SK, Shanely RA, Coombes JS, Koesterer TJ, McKenzie M, Van Gammeren D, Cicale M, Dodd SL: Mechanical ventilation results in progressive contractile dysfunction in the diaphragm. J Appl Physiol 2002, 92: 1851-1858.View ArticlePubMed
- Zhu E, Sassoon CS, Nelson R, Pham HT, Zhu L, Baker MJ, Caiozzo VJ: Early effects of mechanical ventilation on isotonic contractile properties and MAF-box gene expression in the diaphragm. J Appl Physiol 2005, 99: 747-756. 10.1152/japplphysiol.00126.2005View ArticlePubMed
- Radell PJ, Remahl S, Nichols DG, Eriksson LI: Effects of prolonged mechanical ventilation and inactivity on piglet diaphragm function. Intensive Care Med 2002, 28: 358-364. 10.1007/s00134-002-1207-8View ArticlePubMed
- Anzueto A, Peters JI, Tobin MJ, de los Santos R, Seidenfeld JJ, Moore G, Cox WJ, Coalson JJ: Effects of prolonged controlled mechanical ventilation on diaphragmatic function in healthy adult baboons. Crit Care Med 1997, 25: 1187-1190. 10.1097/00003246-199707000-00021View ArticlePubMed
- Testelmans D, Maes K, Wouters P, Powers SK, Decramer M, Gayan-Ramirez G: Infusions of rocuronium and cisatracurium exert different effects on rat diaphragm function. Intensive Care Med 2007, 33: 872-879. 10.1007/s00134-007-0584-4View ArticlePubMed
- Testelmans D, Maes K, Wouters P, Gosselin N, Deruisseau K, Powers S, Sciot R, Decramer M, Gayan-Ramirez G: Rocuronium exacerbates mechanical ventilation induced diaphragm dysfunction in rats. Crit Care Med 2006, 34: 3018-3023.PubMed
- Shanely RA, Zergeroglu MA, Lennon SL, Sugiura T, Yimlamai T, Enns D, Belcastro A, Powers SK: Mechanical ventilation-induced diaphragmatic atrophy is associated with oxidative injury and increased proteolytic activity. Am J Respir Crit Care Med 2002, 166: 1369-1374. 10.1164/rccm.200202-088OCView ArticlePubMed
- DeRuisseau KC, Kavazis AN, Deering MA, Falk DJ, Van Gammeren D, Yimlamai T, Ordway GA, Powers SK: Mechanical ventilation induces alterations of the ubiquitin-proteasome pathway in the diaphragm. J Appl Physiol 2005, 98: 1314-1321. 10.1152/japplphysiol.00993.2004View ArticlePubMed
- Maes K, Testelmans D, Powers S, Decramer M, Gayan-Ramirez G: Leupeptin inhibits ventilator-induced diaphragm dysfunction in rats. Am J Respir Crit Care Med 2007, 175: 1134-1138. 10.1164/rccm.200609-1342OCView ArticlePubMed
- McClung JM, Kavazis AN, Whidden MA, DeRuisseau KC, Falk DJ, Criswell DS, Powers SK: Antioxidant administration attenuates mechanical ventilation-induced rat diaphragm muscle atrophy independent of protein kinase B (PKB Akt) signalling. J Physiol 2007, 585: 203-215. 10.1113/jphysiol.2007.141119PubMed CentralView ArticlePubMed
- McClung JM, Whidden MA, Kavazis AN, Falk DJ, Deruisseau KC, Powers SK: Redox regulation of diaphragm proteolysis during mechanical ventilation. Am J Physiol Regul Integr Comp Physiol 2008, 294: R1608-R1617.View ArticlePubMed
- Powers SK, Kavazis AN, McClung JM: Oxidative stress and disuse muscle atrophy. J Appl Physiol 2007, 102: 2389-2397. 10.1152/japplphysiol.01202.2006View ArticlePubMed
- Clark BC, Fernhall B, Ploutz-Snyder LL: Adaptations in human neuromuscular function following prolonged unweighting: I. Skeletal muscle contractile properties and applied ischemia efficacy. J Appl Physiol 2006, 101: 256-263. 10.1152/japplphysiol.01402.2005View ArticlePubMed
- Powers SK, Kavazis AN, DeRuisseau KC: Mechanisms of disuse muscle atrophy: role of oxidative stress. Am J Physiol Regul Integr Comp Physiol 2005, 288: R337-R344.View ArticlePubMed
- Jaber S, Sebbane M, Koechlin C, Hayot M, Capdevila X, Eledjam JJ, Prefaut C, Ramonatxo M, Matecki S: Effects of short vs. prolonged mechanical ventilation on antioxidant systems in piglet diaphragm. Intensive Care Med 2005, 31: 1427-1433. 10.1007/s00134-005-2694-1View ArticlePubMed
- Falk DJ, Deruisseau KC, Van Gammeren DL, Deering MA, Kavazis AN, Powers SK: Mechanical ventilation promotes redox status alterations in the diaphragm. J Appl Physiol 2006, 101: 1017-1024. 10.1152/japplphysiol.00104.2006View ArticlePubMed
- Zergeroglu MA, McKenzie MJ, Shanely RA, Van Gammeren D, DeRuisseau KC, Powers SK: Mechanical ventilation-induced oxidative stress in the diaphragm. J Appl Physiol 2003, 95: 1116-1124.View ArticlePubMed
- Betters JL, Criswell DS, Shanely RA, Van Gammeren D, Falk D, Deruisseau KC, Deering M, Yimlamai T, Powers SK: Trolox attenuates mechanical ventilation-induced diaphragmatic dysfunction and proteolysis. Am J Respir Crit Care Med 2004, 170: 1179-1184. 10.1164/rccm.200407-939OCView ArticlePubMed
- Van Gammeren D, Falk DJ, Deering MA, Deruisseau KC, Powers SK: Diaphragmatic nitric oxide synthase is not induced during mechanical ventilation. J Appl Physiol 2007, 102: 157-162. 10.1152/japplphysiol.00043.2006View ArticlePubMed
- Whidden MA, McClung JM, Falk DJ, Hudson MB, Smuder AJ, Nelson WB, Powers SK: Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction. J Appl Physiol 2009, 106: 385-394. 10.1152/japplphysiol.91106.2008PubMed CentralView ArticlePubMed
- McClung JM, Van Gammeren D, Whidden MA, Falk DJ, Kavazis AN, Hudson MB, Gayan-Ramirez G, Decramer M, DeRuisseau KC, Powers SK: Apocynin attenuates diaphragm oxidative stress and protease activation during prolonged mechanical ventilation. Crit Care Med 2009, 37: 1373-1379. 10.1097/CCM.0b013e31819cef63PubMed CentralView ArticlePubMed
- Kavazis AN, Talbert EE, Smuder AJ, Hudson MB, Nelson WB, Powers SK: Mechanical ventilation induces diaphragmatic mitochondrial dysfunction and increased oxidant production. Free Radic Biol Med 2009, 46: 842-850. 10.1016/j.freeradbiomed.2009.01.002PubMed CentralView ArticlePubMed
- McClung JM, Judge AR, Talbert EE, Powers SK: Calpain-1 is required for hydrogen peroxide-induced myotube atrophy. Am J Physiol Cell Physiol 2009, 296: C363-C371. 10.1152/ajpcell.00497.2008PubMed CentralView ArticlePubMed
- Goldberg AL, Elledge SJ, Harper JW: The cellular chamber of doom. Sci Am 2001, 284: 68-73.View ArticlePubMed
- McClung JM, Kavazis AN, DeRuisseau KC, Falk DJ, Deering MA, Lee Y, Sugiura T, Powers SK: Caspase-3 regulation of diaphragm myonuclear domain during mechanical ventilation-induced atrophy. Am J Respir Crit Care Med 2007, 175: 150-159. 10.1164/rccm.200601-142OCPubMed CentralView ArticlePubMed
- Lecker SH, Solomon V, Mitch WE, Goldberg AL: Muscle protein breakdown and the critical role of the ubiquitin-proteasome pathway in normal and disease states. J Nutr 1999, 129: 227S-237S.PubMed
- Koh TJ, Tidball JG: Nitric oxide inhibits calpain-mediated proteolysis of talin in skeletal muscle cells. Am J Physiol Cell Physiol 2000, 279: C806-C812.PubMed
- Du J, Wang X, Miereles C, Bailey JL, Debigare R, Zheng B, Price SR, Mitch WE: Activation of caspase-3 is an initial step triggering accelerated muscle proteolysis in catabolic conditions. J Clin Invest 2004, 113: 115-123.PubMed CentralView ArticlePubMed
- Bodine SC, Latres E, Baumhueter S, Lai VK, Nunez L, Clarke BA, Poueymirou WT, Panaro FJ, Na E, Dharmarajan K, Pan ZQ, Valenzuela DM, DeChiara TM, Stitt TN, Yancopoulos GD, Glass DJ: Identification of ubiquitin ligases required for skeletal muscle atrophy. Science 2001, 294: 1704-1708. 10.1126/science.1065874View ArticlePubMed
- Sandri M, Sandri C, Gilbert A, Skurk C, Calabria E, Picard A, Walsh K, Schiaffino S, Lecker SH, Goldberg AL: Foxo transcription factors induce the atrophy-related ubiquitin ligase atrogin-1 and cause skeletal muscle atrophy. Cell 2004, 117: 399-412. 10.1016/S0092-8674(04)00400-3PubMed CentralView ArticlePubMed
- Sassoon CS, Zhu E, Caiozzo VJ: Assist-control mechanical ventilation attenuates ventilator-induced diaphragmatic dysfunction. Am J Respir Crit Care Med 2004, 170: 626-632. 10.1164/rccm.200401-042OCView ArticlePubMed
- Gayan-Ramirez G, Testelmans D, Maes K, Rácz GZ, Cadot P, Zádor E, Wuytack F, Decramer M: Intermittent spontaneous breathing protects the rat diaphragm from mechanical ventilation effects. Crit Care Med 2005, 33: 2804-2809. 10.1097/01.CCM.0000191250.32988.A3View ArticlePubMed
- Futier E, Constantin JM, Combaret L, Mosoni L, Roszyk L, Sapin V, Attaix D, Jung B, Jaber S, Bazin JE: Pressure support ventilation attenuates ventilator-induced protein modifications in the diaphragm. Crit Care 2008, 12: R116. 10.1186/cc7010PubMed CentralView ArticlePubMed
- Sieck GC, Mantilla CB: Effects of mechanical ventilation on the diaphragm. N Engl J Med 2008, 358: 1392-1393. 10.1056/NEJMe0801226View ArticlePubMed
- Caruso P, Denari SD, Ruiz SA, Bernal KG, Manfrin GM, Friedrich C, Deheinzelin D: Inspiratory muscle training is ineffective in mechanically ventilated critically ill patients. Clinics 2005, 60: 479-484. 10.1590/S1807-59322005000600009View ArticlePubMed
- Amaya-Villar R, Garnacho-Montero J, Garcia-Garmendia JL, Madrazo-Osuna J, Garnacho-Montero MC, Luque R, Ortiz-Leyba C: Steroid-induced myopathy in patients intubated due to exacerbation of chronic obstructive pulmonary disease. Intensive Care Med 2005, 31: 157-161. 10.1007/s00134-004-2509-9View ArticlePubMed
- De Jonghe B, Sharshar T, Lefaucheur JP, Authier FJ, Durand-Zaleski I, Boussarsar M, Cerf C, Renaud E, Mesrati F, Carlet J, Raphaël JC, Outin H, Bastuji-Garin S, Groupe de Réflexion et d'Etude des Neuromyopathies en Réanimation: Paresis acquired in the intensive care unit: a prospective multicenter study. JAMA 2002, 288: 2859-2867. 10.1001/jama.288.22.2859View ArticlePubMed
- Maes K, Testelmans D, Cadot P, Deruisseau K, Powers SK, Decramer M, Gayan-Ramirez G: Effects of acute administration of corticosteroids during mechanical ventilation on rat diaphragm. Am J Respir Crit Care Med 2008, 178: 1219-1226. 10.1164/rccm.200702-296OCPubMed CentralView ArticlePubMed
- Tom L, Zhu E, Pham TH, Jiao G, Caiozzo VJ, Sassoon CSH: Effects of methylprednisolone on diaphragmatic contractile properties during mechanical ventilation. Proc Am Thoracic Soc 2006, 3: A137.