Skip to main content

Volume 16 Supplement 3

Sepsis 2012

  • Poster presentation
  • Open access
  • Published:

Noradrenergic neurons regulate the egress and trafficking of splenic monocytes and influence mortality during Gram-negative infection in mice

Background

Neurotransmitters derived from the autonomic nervous system can regulate inflammatory cytokine secretion. This has been extensively studied in the parasympathetic nervous system, where acetylcholine regulates the secretion of TNF from splenic macrophages during LPS-induced inflammation. However, the role of noradrenergic neurons during mouse models of infection has not been characterized. The goal of these experiments was to study the influence of noradrenergic neurons on the immune response during Gram-negative septic peritonitis in mice.

Methods

Peripheral noradrenergic nerves were ablated using 6-hydroxydopamine (6-OHDA), a commonly employed method for studying noradrenergic neurons. Four days later, septic peritonitis was induced by i.p. injection of 150 CFU Klebsiella pneumoniae. Survival, serum and peritoneal bacterial loads, inflammatory cytokine production and leukocyte recruitment were studied at multiple time points after infection. To assess the importance of the NE containing splenic nerve, survival experiments on splenectomized mice with or without 6-OHDA treatment were performed.

Results

Ablation of noradrenergic nerves improved survival following K. pneumoniae septic peritonitis (Figure 1A). Mice in which noradrenergic nerves had been ablated showed a more robust immune response 4 hours after infection with higher systemic IL-6, higher intraperitoneal MCP-1 and a fourfold increase in monocyte recruitment into the peritoneum. Bacterial loads were lower 24 and 48 hours after infection in mice in which noradrenergic nerves had been ablated (Figure 1B). Four hours after infection, 6-OHDA-treated mice recruited more inflammatory monocytes to the infected peritoneum (Figure 1D, CTRL vs. 6-OHDA). Splenectomy prior to noradrenergic nerve ablation abrogated the beneficial effects of 6-OHDA during infection (Figure 1C) and reduced the recruitment of monocytes to the infected peritoneum (Figure 1D, 6-OHDA vs. SPLX + 6-OHDA).

Figure 1
figure 1

Nonadrenergic nerve ablation with 6-OHDA improves survival during K. pneumonia intraperitoneal sepsis (A). Nonadrenergic nerve ablation leads to improved bacterial killing (B) and enhanced monocyte recruitment 4 hours after infection (D). The survival benefit of 6-OHDA treatment required the spleen because splenectomy (SPLX) prior to 6-OHDA treatment abolishes this survival benefit (C). Peritoneal monocytes were quantified 4 hours after infection in 6-OHDA-treated mice with or without spleens (D). 6-OHDA treatment enhanced the recruitment of splenic monocytes to the peritoneum during infection (D). *P < 0.05.

Conclusion

These results suggest that splenic nerve-derived catecholamines regulate the egress of splenic monocytes during infection and that altering this pathway can alter survival during septic peritonitis in mice. These data highlight the emerging role of splenic monocytes during inflammation and infection [1] and add to the body of evidence that the immunosuppressive effects of catecholamines can impair innate immune responses during infection [2]. These experiments may have important implications for patients receiving vasopressors in the ICU.

References

  1. Swirski FK, Nahrendorf M, Etzrodt M, et al.: Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science 2009, 325: 612-616. 10.1126/science.1175202

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Wong CH, Jenne CN, Lee WY, Leger C, Kubes P: Functional innervation of hepatic iNKT cells is immunosuppressive following stroke. Science 2011, 334: 101-105. 10.1126/science.1210301

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Cite this article

Seeley, E., Barry, S., Matthay, M. et al. Noradrenergic neurons regulate the egress and trafficking of splenic monocytes and influence mortality during Gram-negative infection in mice. Crit Care 16 (Suppl 3), P71 (2012). https://doi.org/10.1186/cc11758

Download citation

  • Published:

  • DOI: https://doi.org/10.1186/cc11758

Keywords